<p><b>Copyright information:</b></p><p>Taken from "Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-κB in immortalized and malignant oral keratinocytes"</p><p>http://www.biomedcentral.com/1471-2407/7/176</p><p>BMC Cancer 2007;7():176-176.</p><p>Published online 13 Sep 2007</p><p>PMCID:PMC2078595.</p><p></p> IHOK and HN12 cells were treated with DFO (1.0 mM) for 16 h to allow accumulation of IL-8 mRNA. Cells were then treated with the mRNA synthesis inhibitor actinomycin D (ActD; 5 μg/ml), and either ERK pathway inhibitor (PD98059) or p38 inhibitor (SB203580). GAPDH mRNA was used as an endogenous control message. Same procedure as described in the legend to Fig. 1 was performed. Results are representative of three independent experiments
