<p><b>Copyright information:</b></p><p>Taken from "Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-κB in immortalized and malignant oral keratinocytes"</p><p>http://www.biomedcentral.com/1471-2407/7/176</p><p>BMC Cancer 2007;7():176-176.</p><p>Published online 13 Sep 2007</p><p>PMCID:PMC2078595.</p><p></p> Cells were incubated with DFO (1.0 mM) or IL1-β (10 ng/ml) for the indicated time periods. Levels of IL-8 protein and mRNA were determined by ELISA and semiquantitative RT-PCR, respectively. Numbers below the gels represent the intensity of IL-8 mRNA relative to GAPDH mRNA. These data are representative of three independent experiments