<p><b>Copyright information:</b></p><p>Taken from "Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization"</p><p>http://www.biomedcentral.com/1471-213X/7/102</p><p>BMC Developmental Biology 2007;7():102-102.</p><p>Published online 12 Sep 2007</p><p>PMCID:PMC2080637.</p><p></p>integrin GoH3 (e, f) or anti-β1 integrin MB1.2 antibodies (g, h), then with Alexa Fluor488 (green) or 594 (red) goat anti-rat IgG respectively and analyzed by confocal microscopy as described in Material and methods. These molecules were distributed homogeneously with fine punctuations around the surface of the zona-intact oocytes (e, g), but both formed patches on the acid Tyrode zona-free oocyte surface (f, h). About 90 sections, representing nearly half of the eggs were collected. The maximum projection function was then used to superimpose the different sections. Transmission images corresponding to each oocyte are shown (a, b, c, and d). Bar = 20 μm
