<p><b>Copyright information:</b></p><p>Taken from "Association with the origin recognition complex suggests a novel role for histone acetyltransferase Hat1p/Hat2p"</p><p>http://www.biomedcentral.com/1741-7007/5/38</p><p>BMC Biology 2007;5():38-38.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2140264.</p><p></p>s either labeled with P (panels A and C) or visualized by ethidium bromide (panels B and D). (A) Binding of Hat1-TAP and Cdc45-TAP to and to control sequence at the time of origin firing and later in S-phase. Strains were (BSY679) and (BSY680). One representative experiment with input and immunoprecipitate and the percentage of precipitated DNA is shown. (B) Recruitment of Hat1p to is coincident with Cdc45p and dependent on functional ORC. Strains used for chromatin immunoprecipitation are , , and (BSY699). Strains were held in a-factor at 36°C to inactivate and then released at 23°C. (C) Recruitment of Hat1p (Hat1-TAP) to late replication origin and comparison with sequence. (D) Recruitment of Hat1p to is affected by the allele. Strains used for chromatin immunoprecipitation are , , and (BSY734)
