<p><b>Copyright information:</b></p><p>Taken from "Rapid spread of mouse mammary tumor virus in cultured human breast cells"</p><p>http://www.retrovirology.com/content/4/1/73</p><p>Retrovirology 2007;4():73-73.</p><p>Published online 11 Oct 2007</p><p>PMCID:PMC2169256.</p><p></p>) or absence (B) of 10M DEX. Genomic DNA was extracted from the infected cells at the indicated time points and semiquantitative PCR was performed. NC: non-transduced HS578T cells. PC: second-round infected Hs578T cells. Equal DNA loading was controlled in a PCR assay with GAPDH-specific primers (bottom panels). M: 1 kb marker. (C) Real-time TaqMan PCR quantifying proviral loads in the infected Hs578T cells during the time-course experiment. (D) Equal loading of the PCR reactions was controlled in a Real-time TaqMan PCR specific for GAPDH gene. (E) The viral RNA was quantified by Real-time RT-PCR in cell culture fluids of the infected Hs578T cells grown either in the presence or absence of 10M DEX
