Binding of N-terminal transposase derivatives to the ISY inverted repeat

Abstract

<p><b>Copyright information:</b></p><p>Taken from " transposition of ISY, a bacterial insertion sequence belonging to the Tc/ family"</p><p></p><p>Molecular Microbiology 2007;65(6):1432-1443.</p><p>Published online Jan 2007</p><p>PMCID:PMC2170065.</p><p>© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd</p> A. Transposase deletion derivatives were purified by metal affinity chromatography and analysed by Coomassie-stained Tris-tricine SDS-PAGE. B. The indicated protein (1.25 μM) was incubated with IRL58, and protein–DNA complexes were separated by non-denaturing polyacrylamide gel electrophoresis. C. The indicated concentrations of Tnp and Tnp were incubated with IRL58 and complexes were separated as in (B)