Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of and its role in intracellular replication-8
R infection. Antibodies against were used before permeabilization with saponin in combination with a secondary antibody conjugated with Cy5 and, after permeabilization, with a secondary TRITC-conjugated antibody. Intracellular bacteria were therefore red while extracellular bacteria were expected to be purple (the combination of TRITC and Cy5 signal). In the images all bacteria were intracellular. To detect cellular markers we used anti-LAMP1 and a secondary antibody conjugated with FITC.<p><b>Copyright information:</b></p><p>Taken from "Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of and its role in intracellular replication"</p><p>http://www.biomedcentral.com/1471-2180/8/131</p><p>BMC Microbiology 2008;8():131-131.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2527323.</p><p></p
