<p><b>Copyright information:</b></p><p>Taken from "The zinc finger domain of Wilms' tumor 1 suppressor gene (WT1) behaves as a dominant negative, leading to abrogation of WT1 oncogenic potential in breast cancer cells"</p><p>http://breast-cancer-research.com/content/9/4/R43</p><p>Breast cancer research : BCR 2007;9(4):R43-R43.</p><p>Published online 16 Jul 2007</p><p>PMCID:PMC2206716.</p><p></p> The MCF-7 cells were transiently co-transfected with the reporter constructs (either pGL2 vector, XNM/Luc or Xmut/Luc), with expression plasmids of Wilms' tumor 1 suppressor gene (WT1) isoforms (either pcDNAvector, A, B, C or D) and with WT1–zinc finger domain (ZF) (either pcDNAcontrol (CON) or ZF lacking the lysine–threonine–serine insert (ZF-KTS)) as well as β-galactosidase expression plasmid, respectively. Relative luciferase activity (vertical axis) is indicated. Histograms show average of three independent experiments
