Purification of DTT/urea-disaggregated PP7 and 2PP7 coat protein by gel filtration chromatography in 10 mM acetic acid, 50 mM NaCl (about pH 4)

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Stability and assembly of bacteriophage PP7 virus-like particles"</p><p>http://www.jnanobiotechnology.com/content/5/1/10</p><p>Journal of Nanobiotechnology 2007;5():10-10.</p><p>Published online 26 Nov 2007</p><p>PMCID:PMC2211308.</p><p></p> To estimate the molecular weight of the putative dimer species, bovine serum albumin (68 kD), ovalbumin (43 kD), chymotrypsinogen (25.7 kD) and hen lysozyme (14.4 kD) were used as standards. They peaked at fractions 14, 18, 22 and 26 respectively