<p><b>Copyright information:</b></p><p>Taken from "The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G"</p><p>http://www.retrovirology.com/content/4/1/81</p><p>Retrovirology 2007;4():81-81.</p><p>Published online 24 Nov 2007</p><p>PMCID:PMC2222665.</p><p></p>ith and without Peptide 2 treatments (50 μM), were normalized for p24 and sedimented through a sucrose cushion as described in Methods. The resultant pellets were lysed and resolved via SDS-PAGE and western blotted for A3G and p24. P24 was re-probed in the western blot in addition to the p24 ELISA quantification to validate the normalization. () Virion samples harvested from the co-transfection were normalized for p24 and infected into JC53-bl cells to quantify infectivity by luminescence analysis as described in Methods. Bars represent standard deviations with an n = 3
