Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord-0
<p><b>Copyright information:</b></p><p>Taken from "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord"</p><p>http://www.biomedcentral.com/1472-6750/7/90</p><p>BMC Biotechnology 2007;7():90-90.</p><p>Published online 14 Dec 2007</p><p>PMCID:PMC2225398.</p><p></p>pulations. CMV: Cytomegalovirus immediate early promotor + enhancer. EGFP: enhanced green fluorescent protein. pA: SV40 early mRNA polyadenylation signal. NT3: neurothrophin-3. IRES: internal ribosome entry site. (B) Representative standard PCR and RT-PCR analysis on DNA and mRNA isolated from the different genetically modified hMSC populations used in this study (see numbers below pictures) indicating the presence of transgenic EGFP and/or NT3 DNA and mRNA sequences. M: length marker. GAPDH: glyceraldehyde-3-phosphate dehydrogenase. (C) Representative real-time RT-PCR analysis on mRNA isolated from the different genetically modified hMSC populations used in this study (see numbers below pictures) showing quantitative differences in the level of transgenic EGFP and/or NT3 mRNA transcripts/1000 copies GAPDH; nd: no data available. (D) Representative ELISA measurement on supernatant samples from the different genetically modified hMSC populations used in this study (see numbers below pictures) showing quantitative differences in the level of NT3 secretion in picogram/10cells/24 hours. (E) Representative flow cytometric analysis of EGFP expression by hMSC-EGFP and hMSC-NT3-EGFP populations showing quantitative differences in the level of transgenic EGFP protein expression. SSC: side scatter
