Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins-0
<p><b>Copyright information:</b></p><p>Taken from "Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins"</p><p>http://www.jneuroinflammation.com/content/4/1/30</p><p>Journal of Neuroinflammation 2007;4():30-30.</p><p>Published online 18 Dec 2007</p><p>PMCID:PMC2228280.</p><p></p>(IgG) followed by Alexa 488-conjugated goat anti-mouse IgM and Cy 3-conjugated donkey anti-mouse IgG. Cultures were examined for indirect immunofluoresence employing a Leitz Orthoplan 2 fluorescent microscope. The Th1 treated microglia (ED-1+ cells) have a different appearance when compared to those incubated with additional medium (control). Control microglia do not express MHC class II whereas Th1 treated microglia strongly express MHC class II molecules