The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G-3

Abstract

<p><b>Copyright information:</b></p><p>Taken from "The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G"</p><p>http://www.retrovirology.com/content/4/1/81</p><p>Retrovirology 2007;4():81-81.</p><p>Published online 24 Nov 2007</p><p>PMCID:PMC2222665.</p><p></p> with or without A3G (as indicated below each histogram). During the incubation period, Peptide 2 was dosed into the specified samples (50 μM final concentration). The viral particles collected from the cell culture media over 48 h were normalized for their p24 content and incubated with JC53-bl cells for analysis of infectivity corresponding to luminescence as described in Methods. Infectivity of +Vif virions is shown as percent of the infectivity measured for +Vif/-hA3G/-peptide condition (14,498 red units). The (-) A3G/ΔVif virus control virions lacked Vif and the cells did not express A3G. Infectivity of the ΔVif virions is shown as percent of the infectivity measured with ΔVif/-hA3G/-peptide conditions (5,827 red units). The error bars represent the standard deviation with n = 3

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