nucleofection. Cell lysates were prepared 24 and 48 h after transfection and mRNA and protein expression were analyzed by RT-PCR (A) and Western blot analysis (B). As control, cells were transfected with a control siRNA pool (Ctrl). (A) RT-PCR results of transfected T cells determined 24 h after nucleofection with Control ("siCtrl"), ZAP70 ("siZAP70"), PLC-γ ("siPLC-γ1") and Lck ("siLck") specific siRNAs. (B) Western blot analysis of transfected T cells 48 h after nucleofection. Protein knockdown of ZAP70, PLC-γ and Lck was detected by specific antibodies. β-actin was used as loading control. Shown are the mean values of triplicates of a representative experiments of two independent experiments.<p><b>Copyright information:</b></p><p>Taken from "RNA interference-mediated gene silencing in murine T cells: and validation of proinflammatory target genes"</p><p>http://www.biosignaling.com/content/6/1/3</p><p>Cell communication and signaling : CCS 2008;6():3-3.</p><p>Published online 6 Aug 2008</p><p>PMCID:PMC2517589.</p><p></p
