(A and B) Relative increases (/wt, when wt) or decreases (wt/, when wt) of gene expression were plotted versus their distance to the closest telomere (A) or centromere (B)
A sliding window of 100 genes was used. The average of the 100 genes was used for the y axis, and the distance of the central gene in the window was used for the x axis. The bottom panel of A is an expanded view of the top graph showing the ∼27-kb region close to the telomeres, which exhibits a misregulation in the deletion strain. (C) Expression levels of mRNAs in wt and Δ cells. Total RNA of wt and Δ cells grown in HP and LP was prepared, and cDNA was analyzed by quantitative RT-PCR using specific primers for , , and . Each gene was assayed in triplicates. The mRNA levels of wt HP expression are set as one. One representative dataset of five times independently isolated RNA is shown. Error bars represent SD.<p><b>Copyright information:</b></p><p>Taken from "The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression"</p><p></p><p>The Journal of Cell Biology 2008;182(5):897-910.</p><p>Published online 8 Sep 2008</p><p>PMCID:PMC2528585.</p><p></p
