<p><b>Copyright information:</b></p><p>Taken from "Discovery of novel alternatively spliced transcripts by computational analysis of SAGE data"</p><p>http://www.biomedcentral.com/1471-2164/8/447</p><p>BMC Genomics 2007;8():447-447.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2216036.</p><p></p>ice junctions as shown. Introns and flanking 13 bp sequences were used for extraction of SAGE tags identifying cases of intron retention. For exon skipping validation, one of the primers spanned the predicted splice junction, so that hybridization with the template mRNA was possible only if the predicted transcript was present in the mRNA pool. Detection of an RT-PCR product with a larger size than expected for a normally spliced isoform confirmed intron retention events
