Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells-5

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells"</p><p>http://www.virologyj.com/content/4/1/102</p><p>Virology Journal 2007;4():102-102.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2241602.</p><p></p>ed into an artificial vRNA-CAT reporter construct. These constructs were individually combined with expression plasmids for PB1, PB2, PA and NP proteins and transfected into MDCK cells. At 44 hrs after transfection, cell lysates were analyzed for CAT expression by a colorimetric ELISA assay. In the plasmid pHW72-CAT, the human RNA pol I promoter directs transcription of a negative sense CAT gene