Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells-9

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells"</p><p>http://www.virologyj.com/content/4/1/102</p><p>Virology Journal 2007;4():102-102.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2241602.</p><p></p>oducts and P ΦX174 size marker DNAs (lane M) were subjected to electrophoresis on a 6% polyacrylamide, 7 M urea gel followed by detection of the radioisotope in the gel with a BioRad Molecular Imager Fx. The maximum length of the observed products were approximately 370 bases and 220 bases, respectively, for the reactions using PrimEx1 (lane 1) and PrimEx2 (lane 2). (B) The products from the PrimEx2 reaction were subjected to electrophoresis adjacent to a M13 sequencing ladder on a 6% polyacrylamide, 7 M urea sequencing gel in order to more accurately determine the maximum length of the products synthesized. (C) The MDCK DNA sequences adjacent to the positions where the largest PrimEx2 products terminated

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