Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of is partially mediated via a subclass-specific P-box residue-0

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of is partially mediated via a subclass-specific P-box residue"</p><p>http://www.biomedcentral.com/1471-2199/9/2</p><p>BMC Molecular Biology 2008;9():2-2.</p><p>Published online 7 Jan 2008</p><p>PMCID:PMC2225407.</p><p></p>-1 antiserum [31]. B. EMSA of FAX-1 protein binding to DR1A sequences (AAGTCA DR1) and failing to bind to DRNC sequences (AATTCA repeats). Binding could be competed with 10-fold and 100-fold molar excess of unlabeled competitor DR1G and DR1A DNA, but not DRNC DNA (wedges). Similar results (not shown) were obtained using radioactively-labeled DR1G DNA (AGGTCA DR1). C. and D. EMSA of FAX-1 protein binding to DR1T sequences (ATGTCA DR1) and DR1C sequences (ACGTCA DR1), respectively