Respective contributions of and to transforming growth factor-beta-1 (TGF-β1)-induced increase in extracellular inorganic pyrophosphate (ePPi) production
<p><b>Copyright information:</b></p><p>Taken from "Inorganic pyrophosphate generation by transforming growth factor-beta-1 is mainly dependent on ANK induction by Ras/Raf-1/extracellular signal-regulated kinase pathways in chondrocytes"</p><p>http://arthritis-research.com/content/9/6/R122</p><p>Arthritis Research & Therapy 2007;9(6):R122-R122.</p><p>Published online 22 Nov 2007</p><p>PMCID:PMC2246241.</p><p></p> Effect of small interfering RNA (siRNA) on Ank and PC-1 mRNA levels. Rat chondrocytes were transfected with siRNA 24 hours before TGF-β1 stimulation. Total RNA was extracted from rat chondrocytes exposed to 10 ng/mL of TGF-β1 for 12 hours (Ank) or 24 hours (PC-1) and then subjected to real-time polymerase chain reaction analysis. The level of Ank, PC-1, and L27 mRNAs was normalized to that of S29 mRNA and expressed as mean percentages (± SD) over control values. Effect of Ank or PC-1 siRNA on ePPi levels. Shown are levels of ePPi in culture supernatant of rat chondrocytes transfected with siRNA and then stimulated for 12 or 24 hours with 10 ng/mL of TGF-β1. ePPi levels were normalized to the amount of total cell proteins (= 6) and are expressed as mean (± SD) in picomoles per microgram of protein. Statistically significant differences from the control are indicated as *< 0.05 and from TGF-β1-treated cells as #< 0.05
