<p><b>Copyright information:</b></p><p>Taken from "Temporal transcriptomic analysis of the EGD-e σregulon"</p><p>http://www.biomedcentral.com/1471-2180/8/20</p><p>BMC Microbiology 2008;8():20-20.</p><p>Published online 28 Jan 2008</p><p>PMCID:PMC2248587.</p><p></p> Data shown here is representative of three independent biological replicates. (B) Immuno-blot analysis quantifying σfrom EGD-e at different growth phases. Proteins were isolated from cultures of EGD-e grown for 3 h (lane 1), 4 h (lane 2), 8 h (lane 3) and 16 h (lane 4) in BHI at 37°C and σwas detected using rabbit polyclonal anti-serum produced against COL σ. The Δdeletion mutant (lane 5) was used as negative control and COL (lane 6) was used as positive control for specific binding of the antibody. Molecular masses (in kilodaltons) of prestained SDS-PAGE standard marker (Bio-Rad) are indicated on the left and σis marked on the right
