<p><b>Copyright information:</b></p><p>Taken from "Application of phage display to high throughput antibody generation and characterization"</p><p>http://genomebiology.com/2007/8/11/R254</p><p>Genome Biology 2007;8(11):R254-R254.</p><p>Published online 29 Nov 2007</p><p>PMCID:PMC2258204.</p><p></p> The sensitivity limits of 90 antibodies to 9 different antigens were tested using a mix of antigen coated beads of different antigen densities. To summarize these data, the relative median fluorescent intensity on the bead comprising 459,000 antigen copies per bead was calculated (actual values and clone IDs given in Additional data file 2) and the ratio relative to uncoated beads plotted. To further illustrate sensitivity achieved, the identity of the lowest density bead that could be detected is indicated by the data label according to the following guide: 459,000 antigen copies per bead only (diamonds), down to 57,000 antigen copies per bead (square), and down to 18,000 antigen copies per bead (triangles). The antigens were: 1, Efna2; 2, Efna4; 3, Plaur; 4, Alcam; 5, Il13ra1; 6, Sigrr; 7, Ngfr; 8, Cd22; and 9, Vcam1
