Schematic representation showing the age of the embryo at the time of electroporation and the different times after electroporation at which the embryos were fixed to evaluate the phenotype
For each experimental condition, 23 to 48 sections from 2 to 3 electroporated embryos were analyzed and the percentage of sections displaying ectopic P-H3 cells on the transgenic side determined. The corresponding values are reported for each experimental condition. Cross-sections of the spinal cord showing the phenotype 24 h after electroporation at E1.5 (b,c) and E2.5 (d), respectively. Tuj1 (b) marks the differentiating neurons. The arrows in (c,d) mark mitotic cells in ectopic positions. (b-d) Images obtained with an epifluorescent microscope.<p><b>Copyright information:</b></p><p>Taken from "Forcing neural progenitor cells to cycle is insufficient to alter cell-fate decision and timing of neuronal differentiation in the spinal cord"</p><p>http://www.neuraldevelopment.com/content/3/1/4</p><p>Neural Development 2008;3():4-4.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2265710.</p><p></p
