<p><b>Copyright information:</b></p><p>Taken from "Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment"</p><p>http://www.biomedcentral.com/1472-6750/8/3</p><p>BMC Biotechnology 2008;8():3-3.</p><p>Published online 14 Jan 2008</p><p>PMCID:PMC2254390.</p><p></p>ased medium supplemented with 3% FCS. Before seeding the cells were washed in serum-free media to verify the removal of any residual serum. During a period of two weeks the supernatants were collected as indicated and the cells were counted on the same day. The cultures were fed on the 4and 10day and medium was placed in the cultures on day 6. Although the cells in DI culture proliferated normally under these conditions, they failed to produce measurable quantities of antibody. Panel A: IgM production per hybridoma cell in 3% FCS; Panel B: Number of live cells at each antibody titration
