Ks domains 3 and 4, and the C-terminal HA tag. The 73 amino acids sequence (73d) within domain 1 is highlighted in gray. (B-G) H1299 cells engineered to express wild type IRP2 or two independent clones of the above mutants (except ΔD1) were treated overnight (14 h) with 100 μM hemin or 30 μg/ml FAC and lysates were subjected to Western blotting with HA (top) and β-actin (bottom) antibodies. No clones expressing ΔD1 could be isolated.<p><b>Copyright information:</b></p><p>Taken from "Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity"</p><p>http://www.biomedcentral.com/1471-2199/9/15</p><p>BMC Molecular Biology 2008;9():15-15.</p><p>Published online 28 Jan 2008</p><p>PMCID:PMC2267205.</p><p></p
