duplicate samples of subtracted (S1 and S2) or non-subtracted (NS) cDNA of the RGP (WM1552C) and the metastatic (a pool of WM9, WM852, 1205Lu and WM1617) cell lines. PCR-2 represents the PCR product generated after 10 amplification cycles by nested-PCR using a specific primer for each adaptor. Note the difference between the subtracted and non-subtracted profiles.<p><b>Copyright information:</b></p><p>Taken from "Suppression subtractive hybridization profiles of radial growth phase and metastatic melanoma cell lines reveal novel potential targets"</p><p>http://www.biomedcentral.com/1471-2407/8/19</p><p>BMC Cancer 2008;8():19-19.</p><p>Published online 22 Jan 2008</p><p>PMCID:PMC2267200.</p><p></p