P starting from the ATG and a reverse primer corresponding to the 3' end of ATM1 including its stop codon. The size of the expected fragment was 1734 bp. The template DNA was as follows: Lane 1. DNA from transgenic plants expressing GFP-ATM1(IQ-tail). Lane 2. DNA from wt plants. Lane 3. DNA from the plasmid used to generate the transgenic plants. Lane 4. Molecular weight markers. B. Western blot analysis showing sizes and levels of the expressed transgenes: Lane 1. GFP alone. Lane 2. GFP-ATM1(IQ-tail). Detection was performed with anti-GFP antibody.<p><b>Copyright information:</b></p><p>Taken from "Different subcellular localizations and functions of Arabidopsis myosin VIII"</p><p>http://www.biomedcentral.com/1471-2229/8/3</p><p>BMC Plant Biology 2008;8():3-3.</p><p>Published online 8 Jan 2008</p><p>PMCID:PMC2275265.</p><p></p
