Filled circles were used for fitting with function EAvPeak; fitting results are displayed as line graph. Circles marked by an asterisk indicate the 5 point peak. (B) As part A, but at higher magnification on the y axis. Points for definition of background fluorescence are selected as follows: each position i, going down cycle by cycle from the 5 point peak, is checked until the dF values of at least 3 points in the interval i-8 to i-1 surpass the reference level, which is the average dF of points i, i+1, and i+2. The upper limit of the background definition interval, denoted by a filled arrow, corresponds to i; the lower limit, denoted by an open arrow, is at i-8. Finally, slope and offset of the background line is determined by linear regression on the raw fluorescence data at i-8 to i. (C) Corresponding raw fluorescence data. Fitting results from function EAv are displayed as line graph. Open circles represent points that were excluded from fitting. Asterisks and arrows as above.<p><b>Copyright information:</b></p><p>Taken from "Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence"</p><p>http://www.biomedcentral.com/1471-2105/9/95</p><p>BMC Bioinformatics 2008;9():95-95.</p><p>Published online 12 Feb 2008</p><p>PMCID:PMC2276494.</p><p></p
