SYBR green detection. The diagram shows forward primer, LNA hydrolysis probe with 5' fluorophore (filled circle) and 3' quencher (open circle), and the entire amplicon antisense strand, with reverse primer sequence underlined. A 3' phosphate (P) prevents elongation of the probe.<p><b>Copyright information:</b></p><p>Taken from "Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence"</p><p>http://www.biomedcentral.com/1471-2105/9/95</p><p>BMC Bioinformatics 2008;9():95-95.</p><p>Published online 12 Feb 2008</p><p>PMCID:PMC2276494.</p><p></p
