Non-cell autonomous impairment of oligodendrocyte differentiation precedes CNS degeneration in the Zitter rat: Implications of macrophage/microglial activation in the pathogenesis-5
Ence labeling of the subventricular zone (SVZ) surrounding the dorso-lateral corner of the lateral ventricle of wild-type rats, using antibodies against PDGFR-α () and atrn (). Atrn expression was not detected in PDGFR-α-positive cells (). , , Confocal images of the wild-type SVZ immunostained for A2B5 () and atrn (). Cell nuclei were visualized by TOPRO-3 staining (). show an enlarged view of the SVZ. Arrowhead depicts A2B5-positive but atrn-negative OPCs among the densely packed cells in the SVZ. , Atrn-positive cells (DAB, ) in the gray matter (layer V) of the adult cerebral neocortex. Atrn expression was observed in neurons including typical pyramidal neurons. , Atrn-positive cell (DAB, ) in the corpus callosum of the adult forebrain was indicated by . , , Confocal images of double immunofluorescent labeling with antibodies to atrn () and CNPase () in the adult cerebral cortex (layers II-III) () or the internal granule cell layer of the adult cerebellum (), respectively. Atrn expression was observed in many pyramidal or granule neurons, but not in CNPase-positive oligodendrocytes (). , , Immunohistochemical analysis for specificity of anti-atrn antibody in rat brain. Coronal sections through the adult cerebral cortex were immunostained with anti-atrn antibody. Atrn-immunoreactivity in wild-type SD () and () rats. Scale bars: 20 μm (A, B); 100 μm (C, D); 10 μm (E, F); 10 μm (F); 20 μm (G); 100 μm (I, J). , lateral ventricle.<p><b>Copyright information:</b></p><p>Taken from "Non-cell autonomous impairment of oligodendrocyte differentiation precedes CNS degeneration in the Zitter rat: Implications of macrophage/microglial activation in the pathogenesis"</p><p>http://www.biomedcentral.com/1471-2202/9/35</p><p>BMC Neuroscience 2008;9():35-35.</p><p>Published online 5 Apr 2008</p><p>PMCID:PMC2323389.</p><p></p
