(B) NIH3T3 cells stably expressing GFP-FIP200 were cultured in starvation medium for 120 min and then subjected to immunofluorescence microscopy using anti-Atg16L1 antibody and Alexa Fluor 568–conjugated secondary antibody. Bars, 20 μm. More than 90% of GFP-FIP200 dots were positive for Atg16L1. (C) NIH3T3 cells stably expressing GFP-ULK1 were starved for 120 min and then subjected to immunofluorescence microscopy using anti-FIP200 antibody and Alexa Fluor 568–conjugated secondary antibody. Black squares indicate the enlarged areas shown in insets. Bar, 20 μm.<p><b>Copyright information:</b></p><p>Taken from "FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells"</p><p></p><p>The Journal of Cell Biology 2008;181(3):497-510.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2364687.</p><p></p
