DR3 expression was determined relative to naive CD4 T cell expression levels and normalized to GAPDH levels in all samples. (A) Comparison of DR3 expression on CD4 cells differentiated under Th1, Th2, and Th17 conditions. (B) Time-course analysis of DR3 expression in CD4 T cells differentiating under Th17 conditions. (C) Analysis of DR3 expression in cells differentiated under different combinations of cytokines. Primers used in A–C detect all DR3 isoforms. (D) Analysis of the full-length transmembrane-containing DR3 isoform (variant 1) in Th17 and T reg cells. (E) The ratio of total transmembrane DR3 (variants 1 and 3 combined) and full-length transmembrane DR3 (variant 1) was measured in Th17 and T reg cells. Error bars represent SD.<p><b>Copyright information:</b></p><p>Taken from "TL1A–DR3 interaction regulates Th17 cell function and Th17-mediated autoimmune disease"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1049-1062.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373838.</p><p></p
