Or the indicated time, then stained for GUS activity. (B) Auxin-response defects of lines. Lengths of primary roots of 8-day-old seedlings grown under yellow-filtered light at 22°C on medium supplemented with various concentrations of 2,4-D are shown. Error bars represent standard errors of the means (= 20). (C) 8-day-old Col-0 (Wt) and carrying [16] were heat-shocked for 2 hours, mock (ethanol) treated or treated with 10 μM IBA for 40 minutes, then stained for GUS activity. (D) 8-day-old Col-0 (Wt), , , and carrying [16] were heat shocked for 2 hours. Midway through a 2-hour heat shock, DMSO (mock) or 50 μM MG132 treatment was initiated. Seedlings were stained for GUS activity 2 hours after return to room temperature. Separate experiments revealed that inclusion of DMSO during the heat shock (included as an MG132 carrier in panel D) resulted in more intense AXR3NT-GUS staining (L.C.S., unpublished), which could account for the higher apparent GUS activity in panel D when compared to panel A.<p><b>Copyright information:</b></p><p>Taken from "The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation"</p><p>http://www.biomedcentral.com/1471-2229/8/41</p><p>BMC Plant Biology 2008;8():41-41.</p><p>Published online 18 Apr 2008</p><p>PMCID:PMC2374786.</p><p></p
