F part of the phosphatase catalytic domains of Arabidopsis IBR5 and several putative and confirmed DSP proteins. Sequences shown are the closest IBR5 homologs from rice (Os06g0308100 and Os02g0720300), an IBR5 relative from Arabidopsis with demonstrated DSP activity (At3g23610/DsPTP1; [52]) and three human (Hs) DSP enzymes. Sequences were aligned with the MegAlign program (DNAStar, Madison, WI) using the CLUSTAL W method. Catalytic residues are shaded in purple, conserved DSP signature residues are shaded in blue, residues identical in at least four sequences are shaded in black, similar residues are shaded in gray, and dashes indicate gaps introduced to maximize alignment. (B) Six-week-old Col-0 (Wt), Wt (), Wt (), , (), () line A, and () line B grown in continuous light are shown. (C) Immunoblot analysis with an anti-IBR5 antibody [37]; top panel) and anti-HSC70 antibody (Stressgen Bioreagents) of protein prepared from 2-day-old seedlings of the lines shown in panel B. Positions of IBR5 and HSC70 are indicated at left. (D) Wt () and Wt () display similar 2,4-D response as Wt. Lengths of primary roots of 8-day-old seedlings grown under yellow-filtered light at 22°C on medium supplemented with various concentrations of 2,4-D are shown. Error bars represent standard errors of the means (≥ 18). (E) () line A, and () line B fail to fully rescue 2,4-D resistance. Seedlings were measured as in (D). Error bars represent standard errors of the means (≥ 17). (F) Length of primary roots 4 days after transfer of 4-day-old seedlings to either 0 (ethanol control) or 10 μM ABA medium. Error bars represent standard errors of the means (≥ 8).<p><b>Copyright information:</b></p><p>Taken from "The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation"</p><p>http://www.biomedcentral.com/1471-2229/8/41</p><p>BMC Plant Biology 2008;8():41-41.</p><p>Published online 18 Apr 2008</p><p>PMCID:PMC2374786.</p><p></p
