Cells were incubated for 72 hours with 30 μmol/l ibandronate (Iban) or zoledronic acid (Zole) in culture medium containing 0.6 or 1.6 mmol/l calcium. Apoptotic cell death was assessed by annexin V-phycoerythrin (PE) and 7-amino-actinomycin (7-AAD) double staining. Data are presented as percentages (mean ± standard deviation) of annexin V-PE-positive (AN) and 7-AAD-negative cells. Mean of results pooled from two experiments (= 4). *< 0.01 versus 0.6 mmol/l calcium, analysis of variance, Tukey test.<p><b>Copyright information:</b></p><p>Taken from "Extracellular calcium increases bisphosphonate-induced growth inhibition of breast cancer cells"</p><p>http://breast-cancer-research.com/content/10/1/R4</p><p>Breast Cancer Research : BCR 2008;10(1):R4-R4.</p><p>Published online 11 Jan 2008</p><p>PMCID:PMC2374955.</p><p></p
