Mples (50 ng/μl) were either spiked with 100 pM synthetic let-7b (A549 si, HeLa si, and HT-29 si) or remained non-spiked (A549, HeLa, and HT-29). RT reactions were performed with 50 ng, 25 ng, and 5 ng of all RNA samples, followed by qPCR detection of let-7b in different runs. Columns represent the mean (± SD) of three measurements. A) let-7b quantification by means of the miR-Q approach using both the spike-in controls and the non-spiked samples. B) Quantification of let-7b by means of mirVana™ qRT-PCR using both the spike-in controls and the non-spiked samples.<p><b>Copyright information:</b></p><p>Taken from "miR-Q: a novel quantitative RT-PCR approach for the expression profiling of small RNA molecules such as miRNAs in a complex sample"</p><p>http://www.biomedcentral.com/1471-2199/9/34</p><p>BMC Molecular Biology 2008;9():34-34.</p><p>Published online 10 Apr 2008</p><p>PMCID:PMC2374797.</p><p></p
