-overexpressing lines 7 and 13 (and ) were infiltrated with a suspension of DC3000 (OD= 0.0001 in 10 mM MgCl). Samples were taken at 0 (open bars) or 3 days (closed bars) post inoculation (dpi) to determine the growth of the bacterial pathogen. The means and standard errors colony-forming units (cfu) were calculated from 10 plants for each treatment. Disease symptom development. Pathogen inoculation of wild type, mutants and overexpression lines was performed as in . Pictures of representative inoculated leaves taken at 4 dpi. Pathogen-induced expression. Wild type and -overexpressing plants were infiltrated with a suspension of DC3000 (OD= 0.0001 in 10 mM MgCl). Inoculated leaves were collected at indicated dpi for RNA isolation. RNA gel blot analysis was performed with P-labeled . These experiments were repeated three times with similar results.<p><b>Copyright information:</b></p><p>Taken from "Roles of WRKY3 and WRKY4 Transcription Factors in Plant Responses to Pathogens"</p><p>http://www.biomedcentral.com/1471-2229/8/68</p><p>BMC Plant Biology 2008;8():68-68.</p><p>Published online 20 Jun 2008</p><p>PMCID:PMC2464603.</p><p></p
