(A) A control pollen tube cultured for 2 h, showing the kinked, coiled shape. The arrows indicate the rough surface of the tube. (B) The forepart of an IAA-treated pollen tube cultured for 2 h, showing a smooth, straight shape. (C) The immunosignal of PM H-ATPase in a plasmolized pollen tube, showing fluorescence associated with the plasma membrane, not the cell wall (arrow). The arrowhead indicates the fluorescence associated with the unshed membranes at the apex. (D) Corresponding bright field image of (C). (E, G) Immunofluorescent images of PM H-ATPase in control (E) and IAA-treated (G) tubes. (I) Immunofluorescent images of PM H-ATPase in the middle part of an IAA-treated tube, showing PM H-ATPase accumulates at the tube apex. (F, H, J) Pixel values along the central longitudinal axes of the tubes in (E), (G), and (I), respectively. Bars = 18 μm (A), 13 μm (B), 8 μm (C, D), 6 μm (E, G, I).<p><b>Copyright information:</b></p><p>Taken from "IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in "</p><p></p><p>Journal of Experimental Botany 2008;59(9):2529-2543.</p><p>Published online Jan 2008</p><p>PMCID:PMC2423660.</p><p></p
