Naive CD4 T cells from C57BL/6 mice were stimulated with anti-CD3 mAb/anti-CD28 mAb in the presence of anti–IL-4 mAb and anti–IFN-γ mAb (neutral condition) with 100 ng/ml IL-6 or IL-6 plus 1 ng/ml TGF-β
At indicated times after stimulation, cells were stimulated with PMA/ionomycin, and intracellular staining for the indicated cytokines was performed. Shown are representative FACS profiles from three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Development and characterization of IL-21–producing CD4 T cells"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1369-1379.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413034.</p><p></p
