(A) Purified CD4 T cells from C57BL/6 mice were stimulated with anti-CD3 mAb/anti-CD28 mAb in the presence of anti–IL-4 mAb and anti–IFN-γ mAb with IL-6 for 5 d
Cells were restimulated for another 5 d under the same condition (top) or Th1-, Th2-, or Th17-polarizing conditions (bottom). On day 10, cells were stimulated with anti-CD3 mAb and intracellular staining for indicated cytokines was performed. Shown are representative FACS profiles from three independent experiments. (B) As positive controls, CD4 T cells from C57BL/6 mice were stimulated with anti-CD3 mAb/anti-CD28 mAb under Th1-, Th2-, or Th17-polarizing conditions for 5 d. Cells were restimulated under the same conditions for another 5 d. On day 10, cells were stimulated with anti-CD3 mAb and intracellular staining for indicated cytokines was performed.<p><b>Copyright information:</b></p><p>Taken from "Development and characterization of IL-21–producing CD4 T cells"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1369-1379.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413034.</p><p></p
