Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes-3
RE. The SIV RRE-based packaging system (SIV RRE system) consisted of the packaging plasmid pGP/SIV 1045 RRE and the gene transfer vector pN-EF1α-EGFP/SIV RRE. For production of virus stocks with the SIV RRE-based packaging system either HIV-1 Rev (pCI-HIV Rev) or SIV Rev (pCI-SIV Rev) expression construct was used, as indicated. All transfections also received a VSV-G envelope expression construct (pMD.G) and a HIV-1 Tat (pCMVtat) expression construct. The titers of the vector stocks were determined as described in Materials and Methods. The % of GFP + cells in the absence of pCI-Rev M10 (0 μg) was considered as 100% (Y-axis) for a given packaging system to which other titers obtained at each amount of pCI-Rev M10 were normalized. Increasing amounts of pCI-Rev M10 are depicted on the X-axis. For each transfection, the indicated amount of pCI-Neo was used as a 'filler', to keep the total amount of DNA added at 1.0 μg. The results shown are representative of two independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes"</p><p>http://www.aidsrestherapy.com/content/5/1/11</p><p>AIDS Research and Therapy 2008;5():11-11.</p><p>Published online 5 Jun 2008</p><p>PMCID:PMC2438438.</p><p></p
