The negative control; Lanes 2–4 titration of Tat from 0.01, 0.1 and 1.0 ug to establish a range of activation; Lanes 5–10 are in the presence of 0.1 ug Tat as well as the indicated transfected siRNAs. TZM-bl cells containing an integrated LTR-Luc were transfected with siGFP, siSETDB1, siTIF-1, siG9a, and siHP1 in addition to Tat (0.1 ug) to initiate transcription. Confirmation of the knockdown of SETDB1 is shown in a Western blot below. Each transfection and luciferase assay was repeated at least three times.<p><b>Copyright information:</b></p><p>Taken from "Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR"</p><p>http://www.retrovirology.com/content/5/1/40</p><p>Retrovirology 2008;5():40-40.</p><p>Published online 22 May 2008</p><p>PMCID:PMC2412914.</p><p></p
