Re (HP), the chimeric human globin intron (137 nt), and the primers used for RT-PCR analysis indicated as arrows (see Additional file ). B) RT-PCR from N2A cells transiently transfected with the control vector pRF-Di cis and the pRF-IR2 construct. A 1 kbp DNA ladder is shown on the left side of the gel. NTC, denotes no template control, PCR products generated from plasmid DNA are denoted as plasmid. RT-PCR products are indicated as cDNA. The 18s rRNA control PCR served as internal standard as described previously (49)<p><b>Copyright information:</b></p><p>Taken from "IRES-mediated translation of the carboxy-terminal domain of the horizontal cell specific connexin Cx55.5 in vivo and in vitro"</p><p>http://www.biomedcentral.com/1471-2199/9/52</p><p>BMC Molecular Biology 2008;9():52-52.</p><p>Published online 27 May 2008</p><p>PMCID:PMC2435236.</p><p></p
