G-B and IL-2. Each patient assay was paired with a healthy control. After 12 days of culture, supernatants were harvested. IgG concentration and supernatant reactivity to either PR3 or MPO was measured by ELISA. The amount of IgG detected in the supernatants was of 5.5 ± 2.2 μg mLin the patients compared to 4.1 ± 1.2 μg mLin the control group (. Figure shows the reactivity of the supernatants from PR3ANCA patients towards PR3 antigen. The difference against control individuals was highly significant (P = 0.0082). Figure shows the reactivity of the supernatants from MPOANCA patients towards MPO antigen. The difference was not significant (P = 0.072) although their supernatants showed a clear tendency towards higher reactivity compared to controls. There was no correlation between patients' serum ANCA titre at the time of the assay and their production of ANCA in response to CpG-B as shown in (r= 0.172).<p><b>Copyright information:</b></p><p>Taken from "CpG oligodeoxynucleotide stimulates production of anti-neutrophil cytoplasmic antibodies in ANCA associated vasculitis"</p><p>http://www.biomedcentral.com/1471-2172/9/34</p><p>BMC Immunology 2008;9():34-34.</p><p>Published online 14 Jul 2008</p><p>PMCID:PMC2483256.</p><p></p
