(A) Top kymograph shows sliding of a microtubule (MT) relative to a surface-attached microtubule. Below, the corresponding kymograph of Eg5-GFP shows directional runs between the overlapping microtubules (region marked with two red dotted lines). (B–F) Analysis of Eg5 motility during relative sliding. (B) Scatter plot of all pairs of short-term velocity and diffusion constant determined for a window of 15 s moving over the composite position-time trace of 94 Eg5 motors traced in the overlap zone of 11 microtubule pairs (2,335 points obtained from 2,349 s of total time). The horizontal dotted line indicates the average velocity of sliding microtubules (33 nm/s), and the vertical dotted line indicates the threshold used to discriminate slow and fast diffusion. (C and D) Similar analyses for Eg5 moving on individual microtubules at low ionic strength (C, 70 mM Pipes; ; 4,266 points) and high ionic strength (D, data pooled from 70 mM Pipes + 60 mM KCl and 70 mM Pipes + 80 mM KCl; 2,478 points). (E) Position-time traces. Black, fraction of the composite trace used for B. Green and red, sorted time points with a short-term diffusion constant; < 1,500 nm/s (green) and >1,500 nm/s (red). (F) Histograms of the short-term velocities as obtained from the time points in the green and red trace in E. The arrow indicates the average microtubule sliding velocity. (G) Graph summarizing Eg5 behavior under various conditions. Bar, 2 μm.<p><b>Copyright information:</b></p><p>Taken from "Microtubule cross-linking triggers the directional motility of kinesin-5"</p><p></p><p>The Journal of Cell Biology 2008;182(3):421-428.</p><p>Published online 11 Aug 2008</p><p>PMCID:PMC2500128.</p><p></p
