Of nisin for 1 hour. Culture A was in the presence of nisin all the time; culture B washed and suspended in fresh medium without inducer at hour 1 and 3, and culture C was washed and suspended in fresh medium without inducer only at hour 1. All cultures were grown a total of 6 hours. Protein extracts were analyzed by Western blot at hour 1, 3, and 6. The band in culture B at hour 6 (asterisk) represents the IP-10 "de novo" specifically secreted from hour 3 to 6 in the total absence of nisin demonstrating the ability of to keep expressing and producing IP-10 for at least three hours.<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p
