Compartments labelled by positively charged nanogold during time-course experiments: protoplasts were incubated with 30 nmol of positively charged nanogold and fixed after 5, 15, 30, 45, and 120 min
(A) After 5 min of incubation most of the particles were distributed on the PM and peripheral vesicles (arrows). (B) Some large vesicles near the PM appeared labelled by the probe. (C, D) After 5–120 min of incubation, the probe was seen in small vesicles immediately below the PM (C, see blue arrowheads for peripheral vesicles and black arrowheads for PM) and into inner vesicles in the cortical region of cell (C, D, see black arrows) or deeper in the cytoplasm (C, D, see blue arrows). Golgi bodies appeared labelled by the probe (D, see arrowheads). (E) After 15–120 min of incubation, positively charged nanogold was seen inside large tubular membraneous compartments (large vesicles). (F) Some roundish-tubular vesicles showed internal membranes similar to MVBs labelled by the probe. (G, H, I) The ER (G, arrow) and Golgi bodies (H, I) also contained gold particles. (A, B, E, F, G, H, I) Bar=50 nm. (C, D) Bar=100 nm.<p><b>Copyright information:</b></p><p>Taken from "Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3051-3068.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504345.</p><p></p
