E extracted from control, Se- and Se+ Vero and SK-N-SH cells and GPx1 enzyme activity was measured at days 3, 7 and 10 post-induction of Se deficiency by using the cGPx1 assay kit. Data are reported as mean ± SD of triplicate experiments. * p < 0.05, ** p < 0.005 compared to control cells. Analyses of GPx1 protein by Western blot. 50 μg of total protein extracted from Vero and SK-N-SH cells grown in control, Se- and Se+ media were separated on PAGE, followed by immunoblotting with anti-GPx1. Equal loading was confirmed by re-blotting the same membranes with anti-β-actin.<p><b>Copyright information:</b></p><p>Taken from "effects of selenium deficiency on West Nile virus replication and cytopathogenicity"</p><p>http://www.virologyj.com/content/5/1/66</p><p>Virology Journal 2008;5():66-66.</p><p>Published online 31 May 2008</p><p>PMCID:PMC2453119.</p><p></p
