Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of and its role in intracellular replication-2
Ver and the probe (open rectangle) used in the Southern blot experiments shown in panels B and C. , erythromycin-resistance gene; DUS, DNA uptake sequence; B, HI restriction sites; Hf, fI restriction sites. (B) Southern blot analysis demonstrating the inactivation of . Chromosomal DNAs were extracted from the parental strain B1940 (lane 4) and from three recombinant strains (B1940ΩpriA) transformed with pDEXpriA (lanes 1–3). The fI-restricted chromosomal DNAs were analyzed by Southern blot using the P labeled -specific DNA fragments cloned in pDEXpriA as probes. The arrows on the right indicate -specific fragments whose sizes were deduced on the basis of the relative migration of DNA ladders (bars on the left). (C) Southern blot experiment demonstrating complementation of B1940ΩpriA. B1940ΩpriA was transformed with pACpriA harboring a functional copy of , and chromosomal DNAs were extracted from two recombinant strains (B1940ΩpriA/priA+) (lanes 3 and 4). The DNAs from these two strains, from B1940 (lane 1) and from B1940ΩpriA (lane 2) were digested with fI and analyzed as in panel B.<p><b>Copyright information:</b></p><p>Taken from "Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of and its role in intracellular replication"</p><p>http://www.biomedcentral.com/1471-2180/8/131</p><p>BMC Microbiology 2008;8():131-131.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2527323.</p><p></p
