Mm from bregma) of a 19 week-old mouse after three weeks on dox (0.1 g/l of drinking water), demonstrating absence of GFP-enhanced H1aV fluorescence. White arrowheads point at the position of the CA1 pyramidal cell layer. − + dox indicate the chronology of dox application. (, ) Comparable sections as in and , showing robust re-induction of GFP-enhanced H1aV fluorescence 3 weeks after dox withdrawal. CA1, CA2, and CA3 mark the three morphologically distinct subregions of the hippocampal pyramidal layer. Zero millimeter indicates the position from the bregma. − + − dox explains the chronology of dox application. (, ) Magnification of the CA1 subregion shown in , and . Strong GFP-enhanced H1aV fluorescence was detected in both, the pyramidal cell bodies of the stratum pyramidale and in the dendrites of the stratum radiatum. () Schematic representation of the transgenes driving H1aV expression in the mouse forebrain. The luc and H1aV genes are expressed from a bidirectional transcription unit (upper and lower chart) activated by binding of tTa, expressed under the control of the mouse αCaMKII promoter (middle chart), to the heptamerized tet operator sequences flanked by CMV minimal promoters. Dox application can shut off transgene expression (, , upper chart), which can be re-induced by withdrawal of dox (–, lower chart).<p><b>Copyright information:</b></p><p>Taken from "Select Overexpression of Homer1a in Dorsal Hippocampus Impairs Spatial Working Memory"</p><p></p><p>Frontiers in Neuroscience 2007;1(1):97-110.</p><p>Published online Jan 2007</p><p>PMCID:PMC2518050.</p><p></p
